MersV1, France, Analysis, bibRecord, 000399

Structure of the human lck gene: differences in genomic organisation within src -related genes affect only N-terminal exons

Identifieur interne : 000399 ( France/Analysis ); précédent : 000398; suivant : 000400

Structure of the human lck gene: differences in genomic organisation within src -related genes affect only N-terminal exons

Auteurs : Evelyne Rouer [France] ; Tan Van Huynh [France] ; Sandra Lavareda De Souza [France] ; Marie-Claude Lang [France] ; Sigmund Fischer [France] ; Richard Benarous [France]

Source :

Gene [ 0378-1119 ] ; 1989.

RBID : ISTEX:53F67417DF783D5433D3E05AD4AA47010092C78B

English descriptors

Teeft :
- Analogous position, Biol, Catalytic domains, Cdna, Cdna sequence, Cdna sequences, Clone, Coding exons, Codon, Consensus sequence, Corresponding introns, Different exons, Different members, Different ptks, Encoding, Exon, Exons encoding, First noncoding exon, Further evidence, Garvin, Gene, Genomic, Genomic sequencing, High levels, Homology, Human gene, Human genes, Human genomic library, Human zckgene, Intron, Intron sequences, Kinase, Lghlckl clone, Mosaic proteins, Mouse intron, Mrna, Murine, Murine zckgene, Nucleotide sequence, Nucleotide sequencing, Organisation, Partial restriction, Protein kinase, Ptks, Retroviral promoter insertion, Same position, Second exon, Sequencing, Similar size, Solid lines, Splice, Splice site, Splice sites, Valine residue, Voronova, Zckgene.

Abstract

Abstract: Although cDNA sequences coding for several Rous sarcoma virus Src-related protein tyrosine kinases (PTKs) have been reported for several years, knowledge of the structure and organisation of genes of the src family is still limited. In this work, a detailed structure and organisation of the human lck gene is reported.A 17-kb genomic clone encoding human p56 Lck, a lymphocyte-specific PTK of the Src-related subfamily, has been isolated. The human Ick gene is organized in 13 exons, one more than in the human cellular (c)-src gene. The twelve coding exons are located in this clone, whereas the putative 5'-noncoding exon is probably located very far upstream from the second exon. Splicing sites for exons 4 to 12, which encode both conserved phospholipase-C-like and catalytic domains of the Src-like PTKs, arise exactly at the same position for the human lck, human c-src and c-fgr genes. The only differences concern the splice sites of exons 1' and 2, which encode the unique N-terminal domain of human Lck. These results give further evidence that the different PTKs of the Src-like family have probably evolved through the mechanism of exon shuffling.

Url:

https://api.istex.fr/ark:/67375/6H6-9ZK0HCS1-S/fulltext.pdf

DOI: 10.1016/0378-1119(89)90144-3

Affiliations:

France

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001E03
to stream Istex, to step Curation: 001E03
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to stream Main, to step Exploration: 004B15
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Links to Exploration step

ISTEX:53F67417DF783D5433D3E05AD4AA47010092C78B

Le document en format XML

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<term>Sequencing</term>
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<front><div type="abstract" xml:lang="en">Abstract: Although cDNA sequences coding for several Rous sarcoma virus Src-related protein tyrosine kinases (PTKs) have been reported for several years, knowledge of the structure and organisation of genes of the src family is still limited. In this work, a detailed structure and organisation of the human lck gene is reported.A 17-kb genomic clone encoding human p56 Lck, a lymphocyte-specific PTK of the Src-related subfamily, has been isolated. The human Ick gene is organized in 13 exons, one more than in the human cellular (c)-src gene. The twelve coding exons are located in this clone, whereas the putative 5'-noncoding exon is probably located very far upstream from the second exon. Splicing sites for exons 4 to 12, which encode both conserved phospholipase-C-like and catalytic domains of the Src-like PTKs, arise exactly at the same position for the human lck, human c-src and c-fgr genes. The only differences concern the splice sites of exons 1' and 2, which encode the unique N-terminal domain of human Lck. These results give further evidence that the different PTKs of the Src-like family have probably evolved through the mechanism of exon shuffling.</div>
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Structure of the human lck gene: differences in genomic organisation within src -related genes affect only N-terminal exons

Structure of the human lck gene: differences in genomic organisation within src -related genes affect only N-terminal exons

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri